Ecotoxicology and Environmental Chemistry
Science - 3rd building, 1st floor
Resp: Prof. Francesco Regoli
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Research Activities |
Staff | |
Equipment |
Dott.ssa Maria Elisa Giuliani
Post Doc
071-2204142
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Dott.ssa Marica Mezzelani
PhD
071-2204142
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Dott. Carlo Giacomo Avio
Phd
071-2204142
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Dott. Giuseppe D’Errico
Phd
071-2204142
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Dott. Alessandro Nardi
Phd
071-2204142
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Dott.ssa Marta Di Carlo
Research Fellow
071-2204308
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Equipment | |
The Laboratories of Environmental Chemistry and Ecotoxicology at Department of Life and Environmental Sciences (UPM) are fully equipped for laboratory exposures with marine organisms, chemical analyses of all classes of environmental pollutants and ecotoxicological analyses. Facilities for laboratory experiments with marine organisms consist of approximately 40 aquaria of different volumes for a total of almost 4500 L. Constant conditions are maintained through a recirculating water system and control of temperature.
A new infrastructure for maintenance and experiments with marine organisms is going to be inaugurated, joining a national network of research facilities. This new infrastructure includes a total water volume of more than 35.000 L contained in fiberglass and glass aquaria for approximately 200 indoor mesocosms. The tanks are equipped with modern recirculating, filtration and light systems to reduce power/hydric consumption and the overall Laboratory has been designed to monitor and maintain the major environmental parameters (i.e. temperature, salinity, pH, light) thus allowing different applications and typologies of experiments in controlled conditions.
In addition to basic laboratory instrumentation (including refrigerated centrifuges, thermostatic baths, chemical hoods, ultra low freezers at -80°C, ultrapure water system), laboratory include the following specific instrumentation:
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Instruments for Chemical Analyses
Microwave digestion systems for acid mineralization and organic phase extraction of sediments and biological tissues (CEM, Mars 5 and Mars 6);
Robotic System for automatic solid phase extraction (SPE) Gilson Aspec, with Liquid Handling Gilson Aspec GX-271, and 406 Single Syringe Pump.
Atomic Absorption Spectrophotometer (Varian SpectrAA 220) with automatic Fast Sequential system for electronic control of gas and flame parameters, and VGA 76 for Vapor Generation system;
Atomic Absorption Spectrophotometer with graphite furnace and Zeeman correction (Varian AA240 Zeeman);
Mercury Analyzer, Agilent Cetac Quick Trace M6100
Gas-chromatograph Perkin Elmer Clarus 500 with ECD and FID detectors;
Gas-chromatograph with mass spectrometry detector, auto-sampler and workstation with software GC/MS (Varian Saturn 2100);
Gas-chromatograph with mass spectrometry detector, auto-sampler and workstation with software GC/MS (Agilent Technologies GC 7820A with MSD 5977E);
Liquid chromatograph (HPLC) Perkin Elmer, with binary pump and fluorimetric detector series 200;
Liquid chromatograph HPLC Agilent 1260 Infinity, with quaternary pump, auto-sampler, Diode Array detector 1260 DAD VL, and fluorimetric detector 1260 FLD
Photometer Hach-Lange DR3900, with reader RFID and automatic screening of cuvette kit
Fourier transformed –Infrared Spectrophotometer FT-IR Agilent 660-IR
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Instruments for Ecotoxicological Analyses
Specific instrumentation for ecotoxicological analyses include:
Chromatograph GC Agilent 7820A, with FID detector and Agilent Interface 35900E connected to a Gas-Chromatograph Hewlett-Packard HP 4890 with FID detector for total antioxidant capacity determination;
Temperature-controlled UV-Vis Spectrophotometer, Varian Cary 100 for enzymatic determinations;
Temperature-controlled UV-Vis Spectrophotometer, Varian Cary 50 for enzymatic determinations;
Spectrofluorimeter Varian Eclipse, for cuvettes and plate reader for enzymatic determinations and aromatic metabolites analysis;
Ultracentrifuge Beckman Optima Max-XP;
Electrophoresis systems and gel scan reader for DNA damage analysis;
Real time PCR fully equipped, Applied Biosystems StepOnePlus, and PCR MultiGeneGradient TC 9600-G-230V Labnet International;
Temperature controlled incubator Peltier Memmert Mod. IPP 500
Cryostat Leica CM 1510
Automatic and motorized Vibratome for slice tissues Leica VT 1200S
Fluorescence microscope Olympus BX-51 with digital cooled camera and image analysis system QCapture Pro.
Polarized light microscope Olympus BX-43
Inverted microscope Meiji Techno Model TC5300
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