Ma.S.Bi.C.

NY-MaSBiC
HIGH-THROUGHPUT PROTEIN PRODUCTION FACILITY
The New York - Marche Structural Biology Center is a core facility for High-Throughput Protein Production (HTPP), the high-efficiency production of proteins and membrane proteins, in construction at the DiSVA. This facility is shared with the Dipartimento di Scienze Agrarie, Alimentari ed Ambientali and the Faculty of Medicine and Surgery of the Università Politecnica delle Marche and has been set up within the frame of a strategic partnership with the New York Structural Biology Center (NYSBC, a consortium founded in 2002 by 10 eminent New York academic research institutions, www.nysbc.org). The NY-MaSBiC laboratory will be fully operational at the end of 2014: the laboratory will occupy an area of approximately 200 m2 and will be fully equipped to allow production, expression testing and stability testing for about 500 prokaryotic and 4000-5000 eukaryotic proteins per year. Inside the collaboration with NYSBC, the facility will allow to carry out projects in the biological, biotechnological, pharmaceutical, biomedical, and agricultural fields at national and European level. Moreover, the facility will allow establishing and strengthening collaborations with regional enterprises and Public Health Services; this will facilitate the development of biotechnological science in the Marche region and the founding of start-up enterprises, which will create new jobs in the bio-tech area for young graduates in medical sciences and molecular biology. The new laboratory will also play a key role in the doctoral and postdoctoral training of students and graduates of the Università Politecnica delle Marche.
Equipment
PCR Thermocyclers
Liquind Handler
Incubator for Bacterial, Insect and Mammalian Cells
Centrifuges
Shakers
Sonicators
Microscope with Fluorescence
HPLC/FPLC
Electrophoresis
Project
Ma.S.Bi.C. is situated at the Department of Life and Environmental Sciences.
It consists of two separate labs, one used for the molecular aspect, with robots and automated systems for the amplification and cloning of targets or bacterial cultures, the other set up to host all the equipment for insects and mammals cell cultures.
Pipeline
Target identification
Cloning
Expression on a small scale
Expression on a medium-scale
Screening - Stability
Possible structural determinations
(X-ray crystallography, NMR, FT-IR)
Outlook
Out-Station at Large Scale Facilities (X-Ray - NMR) 
Creation of new start-ups 
Integration of systems for structural analysis 
Selection and production of high interest protein in biotechnology and pharmaceutical industries.
Staff and collaboration
New York Structural Biology Center
Dott.ssa Valentina Pozzi
Dott. Enrico Jr. Baldassarri
Dott.ssa Eleonora Biagetti
Prof. Filippo Mancia Columbia University
(NYSBC)
Via Brecce Bianche
60131 Ancona
Tel.: (+39) 071.220.4991
Fax.: (+39) 071.220.4316
Via Brecce Bianche 10
60131 Ancona
Tel.: (+39) 071.220.4935
Fax.: (+39) 071.220.4685
Via Tronto 10/a
60020 Torrette di Ancona
Tel.: (+39) 071.220.6222
Fax.: (+39) 071.220.6221
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